Alireza Shams; Hossein Jorab Bafan; Mohammadreza Darabi; Fatemeh Haj Manochehri; Amir Haji Abbasi Zarrabi
Volume 22, Issue 2 , May and June 2015, , Pages 532-542
Abstract
Background: Osteoporosis, an erosive bone defect, is one of the major problems among adults over fifty which makes high expenses and many body psychotic and also causes lots of social side effects on people. Antioxidants and some minerals especially trace elements like zinc may be useful as treatment ...
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Background: Osteoporosis, an erosive bone defect, is one of the major problems among adults over fifty which makes high expenses and many body psychotic and also causes lots of social side effects on people. Antioxidants and some minerals especially trace elements like zinc may be useful as treatment for osteoporosis. This study was aimed at evaluating the effects of co-treatment of vitamin E and zinc on bone healing parameters.
Materials & Methods: 39 female Vistar rats with weight range of 180-200 gram were studied. 7 rats were selected as control group and others were ovariectomized and divided to 3 experimental groups: treated with Vit. E daily 200 IU / kg (n=9), treated with zinc daily 22 mg/ lit(n=8) , treated with Vit. E & Zinc(n=9) and finally 8 rat as sham group. After 1 month levels of Calcitonin, Estrogen, Progesterone, Zinc and Calcium in blood samples were measured and evaluated.
Results: Calcitonin levels in blood serum in groups treated with Zinc and Vit. E & Zinc were significant comparing to sham & control groups. Estrogen and Progesterone levels decreased in all experimental groups comparing to control group and occurred changes were significant but Calcium levels in these groups did not show any difference comparing to control and sham groups. Zinc level in serum of all treated groups was altered significantly comparing to control group, although some increased in sham group but not significant.
Conclusion: Our result showed using new treatment methods like Vit. E with zinc could prevent bone loss as the serum index measured changes in treatment of osteoporosis.
Tahmineh Peirouvi; Shiva Roshan Milani; Siamak Salami; Mohammad Ghaderi; Alireza Shams
Volume 21, Issue 3 , July and August 2014, , Pages 461-476
Abstract
Background: Gonadotropin-releasing hormone (GnRH) has been found to be expressed in ovaries in addition to hypothalamus to modulate cell differentiation and induces atretic follicles. Since the death of granulosa cells during the process of follicular atresia occurs by apoptosis phenomenon, in this study, ...
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Background: Gonadotropin-releasing hormone (GnRH) has been found to be expressed in ovaries in addition to hypothalamus to modulate cell differentiation and induces atretic follicles. Since the death of granulosa cells during the process of follicular atresia occurs by apoptosis phenomenon, in this study, we investigated the occurrence of apoptosis of granulosa cells of rat ovarian follicles under the influence of Buserelin acetate, a GnRH agonist.
Materials and Methods: In this experimental case-control study, twelve 25-day-old female Wistar rats were randomly divided into 2 groups. The study and control groups received 0.2 mg/kg/d Buserelin acetate and normal saline, respectively, for 4 days. 24 hours after the last injection, the rats were anesthetized with a lethal dose of chloroform and ovaries were removed. Specimens were fixed in 10% formalin. After the passage, five-micron sections were prepared using a rotary microtome. Measurement of apoptosis was performed using a calibrated light microscope after TUNEL POD staining. Data were analyzed in GraphPad InStat software using independent t-test. P ≤ 0/05 was statistically considered significant.
Results: These data showed the average percentage of apoptotic cells in the control group 2/14 ± 0/52, and in the experimental group 3/75 ± 1/71. This difference was statistically significant (p≤0.0001).
Conclusion: These findings suggest that Buserelin acetate increases apoptosis in the granulosa cells of ovarian follicles.